Haynes Lab:Notebook/Engineering PC-TFs/2013/10/24: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 19: | Line 19: | ||
# <big>''Elute DNA''</big>: '''Transfer the column to a fresh collection tube. Add 100 µl of Elution Solution or molecular biology reagent water to the column.''' For DNA sequencing and other enzymatic applications, use water or 5 mM Tris-HCl, pH 8.0, as an eluant. '''Centrifuge at 12,000 x g for 1 minute.''' The DNA is now present in the eluate and is ready for immediate use or storage at –20 °C. Note: If a more concentrated plasmid DNA preparation is required, the elution volume may be reduced to a minimum of 50 µl. | # <big>''Elute DNA''</big>: '''Transfer the column to a fresh collection tube. Add 100 µl of Elution Solution or molecular biology reagent water to the column.''' For DNA sequencing and other enzymatic applications, use water or 5 mM Tris-HCl, pH 8.0, as an eluant. '''Centrifuge at 12,000 x g for 1 minute.''' The DNA is now present in the eluate and is ready for immediate use or storage at –20 °C. Note: If a more concentrated plasmid DNA preparation is required, the elution volume may be reduced to a minimum of 50 µl. | ||
{| {{table}} | |||
| align="center" style="background:#efefef;"| '''Part''' | |||
| align="center" style="background:#efefef;"|'''Concentration''' | |||
|- | |||
| KAH126||143.967ng/μL | |||
|} | |||
'''Cell Culture of KAH126''' | '''Cell Culture of KAH126''' |
Revision as of 14:03, 25 October 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||
October 24, 2013Sigma-Aldrich Mini-prep Kit
Cell Culture of KAH126 Grow liquid culture of KAH126
|