Haynes Lab:Notebook/Engineering PC-TFs/2013/10/23: Difference between revisions
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==October 23, 2013== | ==October 23, 2013== | ||
'''Transfection of KAH126''' | '''Transfection of KAH126''' | ||
{| {{table}} cellspacing="7" width=700px | |||
|- | |||
| Plate-Well || Plasmid || DNA || Volume|| dH2O|| Lipo || PLUS reagent || <u>Opti-MEM (total)</u> | |||
|- | |||
| 1-1 || BD002 (3:1) + FlpE || 1.5 + 0.5 μg || 8.187 + 2.60 μL || 9.22μL|| 2.5μL|| 7.5 μL || 570 μL | |||
|} | |||
# Prepare DNA-Lipofectamine complexes (600 μl per sample) as follows: | # Prepare DNA-Lipofectamine complexes (600 μl per sample) as follows: |
Revision as of 09:31, 25 October 2013
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October 23, 2013Transfection of KAH126
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