Haynes Lab:Notebook/Engineering PC-TFs/2013/10/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 31: | Line 31: | ||
# Place the new flask into the 37°C incubator. The cells should adhere to the growth surface in a couple of hours. | # Place the new flask into the 37°C incubator. The cells should adhere to the growth surface in a couple of hours. | ||
# Discard the left-over cells as liquid waste by aspirating them into the vacuum trap. Discard all plastics, etc. that have come into contact with growth medium and cells as biohazard waste. | # Discard the left-over cells as liquid waste by aspirating them into the vacuum trap. Discard all plastics, etc. that have come into contact with growth medium and cells as biohazard waste. | ||
#Seed cultures in 6-well plates at ~2.5x105 so that cells will be ~5.0x105 at the time of transfection. | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 09:34, 25 October 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |
October 22, 2013Materials
Procedure
|