Haynes Lab:Notebook/Engineering PC-TFs/2013/10/22: Difference between revisions
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==October 22, 2013== | ==October 22, 2013== | ||
'''Materials''' | |||
* 70% ethanol spray bottle | |||
* 1x Phosphate-buffered saline (PBS) | |||
* Trypsin-EDTA buffer/ media | |||
* [http://openwetware.org/wiki/Haynes:MamCultureMedia Complete cell culture medium] (e.g., 10% FBS, 1% pen-strep) | |||
* Sterile tissue culture-treated vent-capped T-75 flask, one per new culture | |||
* 100% confluent (dense) cell culture (check confluency under the light microscope) | |||
'''Procedure''' | |||
# '''Pre-warm all liquid reagents to 37°C in the bead bath'''. | # '''Pre-warm all liquid reagents to 37°C in the bead bath'''. | ||
# After reagents are warmed, spray bottles down with 70% ethanol and prepare the biosafety hood as for routine work. Do all work in the biosafety hood. | # After reagents are warmed, spray bottles down with 70% ethanol and prepare the biosafety hood as for routine work. Do all work in the biosafety hood. |
Revision as of 09:24, 25 October 2013
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October 22, 2013Materials
Procedure
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