Haynes Lab:Notebook/Engineering PC-TFs/2013/10/21: Difference between revisions
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*Add 400μL of Zyppy Wash Buffer to the column. Centrifuge at top speed for 30 seconds. | *Add 400μL of Zyppy Wash Buffer to the column. Centrifuge at top speed for 30 seconds. | ||
*Transfer column to 1.5 microcentrifuge tube and add 50μL of Zyppy Elution Buffer directly to column. Centrifuge for 1 minute at top speed to elute DNA. | *Transfer column to 1.5 microcentrifuge tube and add 50μL of Zyppy Elution Buffer directly to column. Centrifuge for 1 minute at top speed to elute DNA. | ||
Measure concentration of each fragment: | |||
* Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application | |||
* Go to Nucleic Acid Quantification. | |||
* Pipette 2 µL of each DNA sequence into Take 3 plate. | |||
* Place plate in EPOCH plate reader. | |||
Revision as of 14:08, 25 October 2013
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October 21, 2013Miniprep liquid cultures
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