Haynes Lab:Notebook/Engineering PC-TFs/2013/10/21: Difference between revisions
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==October 21, 2013== | ==October 21, 2013== | ||
<big>''Miniprep liquid cultures''</big> | |||
*Add 100 μL of 7X Lysis Buffer (Blue) to 600 μL of E.coli culture in a 1.5 ml microcentrifuge tube. Mix by inverting tube 4-6times. | *Add 100 μL of 7X Lysis Buffer (Blue) to 600 μL of E.coli culture in a 1.5 ml microcentrifuge tube. Mix by inverting tube 4-6times. | ||
*Add 350 μL of cold Neutralization Buffer (Yellow), mix thoroughly. Neutralization is complete when sample becomes yellow and precipitate has formed. | *Add 350 μL of cold Neutralization Buffer (Yellow), mix thoroughly. Neutralization is complete when sample becomes yellow and precipitate has formed. | ||
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<big>''Measure concentration of each fragment''</big>: | |||
* Load Gen 5 2.0 software. Go to the Task Manager-Take 3 Application | |||
* Go to Nucleic Acid Quantification. | |||
* Pipette 2 µL of each DNA sequence into Take 3 plate. | |||
* Place plate in EPOCH plate reader. | |||
{| {{table}} | |||
| align="center" style="background:#efefef;"| '''Part''' | |||
| align="center" style="background:#efefef;"|'''Concentration''' | |||
|- | |||
| KAH126 (1)|| 22.239 ng/μL | |||
|- | |||
| KAH126 (2)||57.672 ng/μL | |||
|- | |||
| CMV+MV9 (1)||11.323 ng/μL | |||
|- | |||
| CMV+MV9 (2)||1.92 ng/μL | |||
|- | |||
| CMV+MV9 (3)||27.394 ng/μL | |||
|} | |||
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Revision as of 14:13, 25 October 2013
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October 21, 2013Miniprep liquid cultures
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