Haynes Lab:Notebook/Engineering PC-TFs/2013/10/19: Difference between revisions
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== | ==October 19, 2013== | ||
* | ''Bacterial Transformation of CMV Promoter + MV9'' | ||
*Warm 100 μg/mL Amp agar plate at 37°C | |||
*Thaw fresh tube of DH5α Turbo cells on ice | |||
*Add 30 μL of DH5α Turbo Cells to DNA + dH<sub>2</sub>O | |||
*Incubate cells + DNA on ice for 5 minutes. | |||
*Label pre-warmed plate. | |||
*Transfer cells + DNA onto agar. | |||
*Add 8-10 glass beads, shake, then discard beads. | |||
Incubate plates at 37°C overnight and retrieve next day. | |||
Revision as of 14:04, 20 October 2013
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October 19, 2013Bacterial Transformation of CMV Promoter + MV9
Incubate plates at 37°C overnight and retrieve next day.
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