Haynes Lab:Notebook/Engineering PC-TFs/2013/10/19: Difference between revisions
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| align="center" style="background:#efefef;"| '''Part''' | | align="center" style="background:#efefef;"| '''Part''' | ||
| align="center" style="background:#efefef;"|'''Concentration''' | | align="center" style="background:#efefef;"|'''Concentration''' | ||
|- | |- | ||
| CMV (insert)||15.535 ng/μL | | CMV (insert)||15.535 ng/μL | ||
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|} | |} | ||
<span style="font-size: | <span style="font-size:95%">''Calculations'' | ||
<span style="font-size: | <span style="font-size:95%">ng insert = bp insert/bp vector * 2 * 50 <br> | ||
ng insert = 588/4611 * 2 *50 = 12.75211 ng | ng insert = 588/4611 * 2 *50 = 12.75211 ng | ||
<span style="font-size: | <span style="font-size:95%">μL insert = ng insert/concentration insert<br> | ||
μL insert = 12.75211/15.535 = .82μL | μL insert = 12.75211/15.535 = .82μL | ||
<span style="font-size: | <span style="font-size:95%">μL vector = 50 ng/concentration vector<br> | ||
μL vector = 50/20.838 = 2.4 μL | μL vector = 50/20.838 = 2.4 μL | ||
Revision as of 14:20, 20 October 2013
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October 19, 2013Ligation of CMV + MV9
Calculations ng insert = bp insert/bp vector * 2 * 50 μL insert = ng insert/concentration insert μL vector = 50 ng/concentration vector
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