Haynes Lab:Notebook/Engineering PC-TFs/2013/10/05: Difference between revisions
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*Perform restriction digest for CMV promoter | *Perform restriction digest for CMV promoter | ||
Revision as of 14:33, 7 October 2013
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October 5, 2013
PEG/MgCl2 Precipitation
DNA Clean & Concentrator
Gel Electrophoresis
Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to Lis and Schleif 1975, NAR. I kept DNA from the supernatant and discarded the precipitated DNA. Therefore, I should see larger bands at 2% PEG and smaller bands at 15% PEG.
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