Haynes Lab:Notebook/Engineering PC-TFs/2013/10/05: Difference between revisions
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|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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* PEG/MgCl<sub>2</sub> | * PEG/MgCl<sub>2</sub> | ||
** Make 50 mL | ** Make 50 mL | ||
***Put water, then | ***Put water, then MgCl<sub>2</sub>, then PEG 8000 in 50 mL conical tube | ||
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<br> <br> <br> <br> <br> <br> <br> <br> | <br> <br> <br> <br> <br> <br> <br> <br> <br> <br> <br> | ||
*Perform restriction digest for CMV promoter | *Perform restriction digest for CMV promoter | ||
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| PstI|| 1.0 μL | | PstI|| 1.0 μL | ||
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| | | 10x FastDigest buffer || 3μL | ||
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| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 15 μL | ||
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| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. |
Latest revision as of 23:25, 26 September 2017
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October 5, 2013
PEG/MgCl2 Precipitation
DNA Clean & Concentrator
Gel Electrophoresis
Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to Lis and Schleif 1975, NAR. I kept DNA from the supernatant and discarded the precipitated DNA. Therefore, I should see larger bands at 2% PEG and smaller bands at 15% PEG.
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