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Summary Perform restriction digest for CMV promoter
Reagent
Volume
DNA (plasmid)
10 μL
XbaI
1.0 μL
PstI
1.0 μL
Green 10x FastDigest buffer
3.0 μL
dH2 O
15 μL
30 μL --> 37°C/ ~10 min.
Measure the following into 1.5 mL tubes.
Reagent
15%
10%
7%
5%
2%
30% PEG
50 μL
33 μL
23 μL
17 μL
7 μL
DNA
15μL
15 μL
15 μL
15 μL
15 μL
dH2 O
35 μL
52 μL
62 μL
68 μL
78 μL
Total
100 μL
100 μL
100 μL
100 μL
100 μL
