Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 21: | Line 21: | ||
| PstI|| 1.0 μL | | PstI|| 1.0 μL | ||
|- | |- | ||
| Green 10x FastDigest buffer || | | Green 10x FastDigest buffer || 0μL | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 18 μL | ||
|- | |- | ||
| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. |
Revision as of 12:17, 28 September 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||
September 26, 2013Restriction Digest
PEG/MgCl2 Precipitation
DNA Clean & Concentrator
Gel Electrophoresis
Higher molecular mass DNA precipitates at lower PEG concentrations than lower molecular mass DNA, according to Lis and Schleif 1975, NAR. I kept DNA from the supernatant and discarded the precipitated DNA. Therefore, I should see (larger or smaller?) bands at 2% PEG and (larger or smaller?) bands at 15% PEG.
|