Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 29: | Line 29: | ||
''PEG/MgCl<sub>2</sub> Precipitation'' | ''PEG/MgCl<sub>2</sub> Precipitation'' | ||
* This procedure was adapated from [http://openwetware.org/wiki/Protocol_Size_selective_DNA_precipitation_by_PEG/MgCl2 Size selective DNA precipitation] | |||
*Measure the following into 1.5 mL tubes. | *Measure the following into 1.5 mL tubes. | ||
Line 72: | Line 73: | ||
[[Image:hiddendragonsofthetemple.jpg|250px|CMV PEG/MgCl<sub>2</sub> Precipitation 9/26/2013]] | [[Image:hiddendragonsofthetemple.jpg|250px|CMV PEG/MgCl<sub>2</sub> Precipitation 9/26/2013]] | ||
The higher the final PEG concentration, the smaller the fragments that will stay in the supernatant. According to the protocol at [http://openwetware.org/wiki/Protocol_Size_selective_DNA_precipitation_by_PEG/MgCl2 Size selective DNA precipitation] the sizes of the DNA that should remain in the supernatant are: | |||
* 10% PEG, > 300bp in supernatant | |||
* 6% PEG, > 500bp | |||
* 5% PEG, > 700bp | |||
* 4% PEG, > 1kb | |||
Revision as of 10:18, 27 September 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||
September 26, 2013Restriction Digest
PEG/MgCl2 Precipitation
DNA Clean & Concentrator
Gel Electrophoresis
The higher the final PEG concentration, the smaller the fragments that will stay in the supernatant. According to the protocol at Size selective DNA precipitation the sizes of the DNA that should remain in the supernatant are:
|