Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26: Difference between revisions

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(Autocreate 2013/09/26 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
 
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==Summary==
==Summary==
*  
*Measure the following into 1.5 mL tubes.
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''15%'''
| bgcolor=#cfcfcf | '''10%'''
| bgcolor=#cfcfcf | '''7%'''
| bgcolor=#cfcfcf | '''5%'''
| bgcolor=#cfcfcf | '''2%'''
|-
| 30% PEG || 50 μL|| 33 μL || 23 μL|| 17 μL || 7 μL
|-
| DNA|| 15μL||15 μL||15 μL||15 μL||15 μL
|-
| dH<sub>2</sub>O|| 35 μL||52 μL||62 μL||68 μL||78 μL
|-
| Total || 100 μL || 100 μL|| 100 μL|| 100 μL|| 100 μL
|}
 




Revision as of 19:14, 26 September 2013



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Summary

  • Measure the following into 1.5 mL tubes.
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL 15 μL 15 μL 15 μL 15 μL
dH2O 35 μL 52 μL 62 μL 68 μL 78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL



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