Haynes Lab:Notebook/Engineering PC-TFs/2013/09/26

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(Summary)
(Summary)
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==Summary==
==Summary==
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*Perform restriction digest for CMV promoter
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{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
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|- valign="top"
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| bgcolor=#cfcfcf | '''Reagent'''
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| bgcolor=#cfcfcf | '''Volume'''
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|-
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| DNA (plasmid) || 10 μL
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|-
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| XbaI|| 1.0 μL
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|-
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| PstI|| 1.0 μL
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|-
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| Green 10x FastDigest buffer || 3.0 μL
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|-
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| dH<sub>2</sub>O || 15 μL
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|-
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| &nbsp; || 30 μL --> 37°C/ ~10 min.
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|}
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*Measure the following into 1.5 mL tubes.
*Measure the following into 1.5 mL tubes.
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->

Revision as of 22:15, 26 September 2013



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Summary

  • Perform restriction digest for CMV promoter
Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.


  • Measure the following into 1.5 mL tubes.
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL15 μL15 μL15 μL15 μL
dH2O 35 μL52 μL62 μL68 μL78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL



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