Haynes Lab:Notebook/Engineering PC-TFs/2013/09/21

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(Autocreate 2013/09/21 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
(Summary)
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==Summary==
==Summary==
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* Although there was no pellet, ran a DNA Clean & Concentrator on samples.
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**Add 2 volumes of DNA Binder Buffer to each DNA sample (200 μL).
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**Place mixture in spin column into 2 ml collection tube.
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**Centrifuge at full speed for 30 seconds.
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**Add 20 μL of DNA Wash Buffer and centrifuge for 30 seconds. Repeat this step.
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**Place Zymo-Spin Column into a new 1.5 ml tube. Add 20 μL  of water directly to column matrix and spin to elute DNA.

Revision as of 21:23, 26 September 2013



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Summary

  • Although there was no pellet, ran a DNA Clean & Concentrator on samples.
    • Add 2 volumes of DNA Binder Buffer to each DNA sample (200 μL).
    • Place mixture in spin column into 2 ml collection tube.
    • Centrifuge at full speed for 30 seconds.
    • Add 20 μL of DNA Wash Buffer and centrifuge for 30 seconds. Repeat this step.
    • Place Zymo-Spin Column into a new 1.5 ml tube. Add 20 μL of water directly to column matrix and spin to elute DNA.



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