Haynes Lab:Notebook/Engineering PC-TFs/2013/09/21: Difference between revisions
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(Autocreate 2013/09/21 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs) |
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==Summary== | ==Summary== | ||
* | * Although there was no pellet, ran a DNA Clean & Concentrator on samples. | ||
**Add 2 volumes of DNA Binder Buffer to each DNA sample (200 μL). | |||
**Place mixture in spin column into 2 ml collection tube. | |||
**Centrifuge at full speed for 30 seconds. | |||
**Add 20 μL of DNA Wash Buffer and centrifuge for 30 seconds. Repeat this step. | |||
**Place Zymo-Spin Column into a new 1.5 ml tube. Add 20 μL of water directly to column matrix and spin to elute DNA. | |||
Revision as of 18:23, 26 September 2013
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