Haynes Lab:Notebook/Engineering PC-TFs/2013/09/19

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September 20, 2013

  • PEG/MgCl2
    • Make 50 mL
Reagent Volume
PEG 15 g/mL
MgCl2 1.5 mL
H2O 33.5 mL
Total 50 mL


Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL 15 μL 15 μL 15 μL 15 μL
dH2O 35 μL 52 μL 62 μL 68 μL 78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL



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