Haynes Lab:Notebook/Engineering PC-TFs/2013/09/19

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(September 20, 2013)
Current revision (00:26, 22 September 2013) (view source)
(September 20, 2013)
 
Line 49: Line 49:
*Centrifuge  for 15 minutes at 10,000g.
*Centrifuge  for 15 minutes at 10,000g.
 +
*There should be a formation of pellet. I did not see a pellet.

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September 20, 2013

  • PEG/MgCl2
    • Make 50 mL
Reagent Volume
PEG 15 g/mL
MgCl2 1.5 mL
H2O 33.5 mL
Total 50 mL










  • Measure the following into 1.5 mL tubes.
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL15 μL15 μL15 μL15 μL
dH2O 35 μL52 μL62 μL68 μL78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL
  • Centrifuge for 15 minutes at 10,000g.
  • There should be a formation of pellet. I did not see a pellet.



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