Haynes Lab:Notebook/Engineering PC-TFs/2013/09/19

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(September 20, 2013)
(September 20, 2013)
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{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
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| 30% PEG || 50 μL|| 33 μL || 23 μL|| 17 μL || 7 μL
| 30% PEG || 50 μL|| 33 μL || 23 μL|| 17 μL || 7 μL
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| DNA|| 15μL||15 μL||15 μL||15 μL||15 μL||
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| DNA|| 15μL||15 μL||15 μL||15 μL||15 μL
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| dH<sub>2</sub>O|| 35 μL||52 μL||62 μL||68 μL||78 μL||
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| dH<sub>2</sub>O|| 35 μL||52 μL||62 μL||68 μL||78 μL
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| Total || 100 μL || 100 μL|| 100 μL|| 100 μL|| 100 μL
| Total || 100 μL || 100 μL|| 100 μL|| 100 μL|| 100 μL

Revision as of 23:17, 21 September 2013



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September 20, 2013

  • PEG/MgCl2
    • Make 50 mL
Reagent Volume
PEG 15 g/mL
MgCl2 1.5 mL
H2O 33.5 mL
Total 50 mL


Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL15 μL15 μL15 μL15 μL
dH2O 35 μL52 μL62 μL68 μL78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL



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