Haynes Lab:Notebook/Engineering PC-TFs/2013/09/19

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(September 20, 2013)
(September 20, 2013)
Line 27: Line 27:
|50 mL
|50 mL
|-
|-
 +
|}
 +
 +
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
 +
|- valign="top"
 +
| bgcolor=#cfcfcf | '''Reagent'''
 +
| bgcolor=#cfcfcf | '''15%'''
 +
| bgcolor=#cfcfcf | '''10%'''
 +
| bgcolor=#cfcfcf | '''7%'''
 +
| bgcolor=#cfcfcf | '''5%'''
 +
| bgcolor=#cfcfcf | '''2%'''
 +
|-
 +
| 30% PEG || 50 μL|| 33 μL || 23 μL|| 17 μL || 7 μL
 +
|-
 +
| DNA|| 15μL||15 μL||15 μL||15 μL||15 μL||
 +
|-
 +
| dH<sub>2</sub>O|| 35 μL||52 μL||62 μL||68 μL||78 μL||
 +
|-
 +
| Total || 100 μL || 100 μL|| 100 μL|| 100 μL|| 100 μL
 +
|}
|}

Revision as of 00:17, 22 September 2013



Main project page
Previous entry      Next entry

September 20, 2013

  • PEG/MgCl2
    • Make 50 mL
Reagent Volume
PEG 15 g/mL
MgCl2 1.5 mL
H2O 33.5 mL
Total 50 mL
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL15 μL15 μL15 μL15 μL
dH2O 35 μL52 μL62 μL68 μL78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL



Personal tools