Haynes Lab:Notebook/Engineering PC-TFs/2013/09/19: Difference between revisions

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==Summary==
==September 20, 2013==
* PEG/MgCl<sub>2</sub>
** Make 50 mL
 
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{|border="1" cellpadding="6" cellspacing="0" align="left"
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|33.5 mL
|33.5 mL
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|-
|Total
|'''Total'''
|50 mL
|50 mL
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|-
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<br><br><br><br><br> <br> <br> <br> <br>
*Measure the following into 1.5 mL tubes.
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''15%'''
| bgcolor=#cfcfcf | '''10%'''
| bgcolor=#cfcfcf | '''7%'''
| bgcolor=#cfcfcf | '''5%'''
| bgcolor=#cfcfcf | '''2%'''
|-
| 30% PEG || 50 μL|| 33 μL || 23 μL|| 17 μL || 7 μL
|-
| DNA|| 15μL||15 μL||15 μL||15 μL||15 μL
|-
| dH<sub>2</sub>O|| 35 μL||52 μL||62 μL||68 μL||78 μL
|-
| Total || 100 μL || 100 μL|| 100 μL|| 100 μL|| 100 μL
|}
*Centrifuge  for 15 minutes at 10,000g.
*There should be a formation of pellet. I did not see a pellet.




Revision as of 21:26, 21 September 2013



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September 20, 2013

  • PEG/MgCl2
    • Make 50 mL
Reagent Volume
PEG 15 g/mL
MgCl2 1.5 mL
H2O 33.5 mL
Total 50 mL










  • Measure the following into 1.5 mL tubes.
Reagent 15% 10% 7% 5% 2%
30% PEG 50 μL 33 μL 23 μL 17 μL 7 μL
DNA 15μL 15 μL 15 μL 15 μL 15 μL
dH2O 35 μL 52 μL 62 μL 68 μL 78 μL
Total 100 μL 100 μL 100 μL 100 μL 100 μL
  • Centrifuge for 15 minutes at 10,000g.
  • There should be a formation of pellet. I did not see a pellet.



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