Haynes Lab:Notebook/Engineering PC-TFs/2013/09/19: Difference between revisions
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== | ==September 20, 2013== | ||
{|border=" | * PEG/MgCl<sub>2</sub> | ||
** Make 50 mL | |||
{|border="1" cellpadding="6" cellspacing="0" align="left" | |||
|- | |- | ||
! scope="col" style="background:#efefef;" | | ! scope="col" style="background:#efefef;" | Reagent | ||
! scope="col" style="background:#efefef;" | Volume | |||
! scope="col" style="background:#efefef;" | | |||
|- | |- | ||
| | |PEG | ||
| | |15 g/mL | ||
|- | |- | ||
|2 | |MgCl<sub>2</sub> | ||
| | |1.5 mL | ||
|- | |- | ||
| | |H<sub>2</sub>O | ||
|33.5 mL | |||
| | |||
|- | |- | ||
| | |'''Total''' | ||
| | |50 mL | ||
|- | |- | ||
|} | |} | ||
<br><br><br><br><br> <br> <br> <br> <br> | |||
*Measure the following into 1.5 mL tubes. | |||
{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | |||
| bgcolor=#cfcfcf | '''Reagent''' | |||
| bgcolor=#cfcfcf | '''15%''' | |||
| bgcolor=#cfcfcf | '''10%''' | |||
| bgcolor=#cfcfcf | '''7%''' | |||
| bgcolor=#cfcfcf | '''5%''' | |||
| bgcolor=#cfcfcf | '''2%''' | |||
|- | |||
| 30% PEG || 50 μL|| 33 μL || 23 μL|| 17 μL || 7 μL | |||
|- | |||
| DNA|| 15μL||15 μL||15 μL||15 μL||15 μL | |||
|- | |||
| dH<sub>2</sub>O|| 35 μL||52 μL||62 μL||68 μL||78 μL | |||
|- | |||
| Total || 100 μL || 100 μL|| 100 μL|| 100 μL|| 100 μL | |||
|} | |||
*Centrifuge for 15 minutes at 10,000g. | |||
*There should be a formation of pellet. I did not see a pellet. | |||
Revision as of 21:26, 21 September 2013
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September 20, 2013
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