Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17

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(September 17, 2013)
Current revision (00:09, 22 September 2013) (view source)
(September 17, 2013)
 
Line 12: Line 12:
|- valign="top"
|- valign="top"
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Reagent'''
-
| bgcolor=#cfcfcf | '''15%'''
+
| bgcolor=#cfcfcf | '''Volume'''
-
| bgcolor=#cfcfcf | '''10%'''
+
-
| bgcolor=#cfcfcf | '''7%'''
+
-
| bgcolor=#cfcfcf | '''5%'''
+
-
| bgcolor=#cfcfcf | '''2%'''
+
|-
|-
-
| DNA (plasmid) || 10 μL|| || || ||
+
| DNA (plasmid) || 10 μL
|-
|-
-
| XbaI|| 1.0 μL|| || || ||
+
| XbaI|| 1.0 μL
|-
|-
-
| PstI|| 1.0 μL|| || || ||
+
| PstI|| 1.0 μL
|-
|-
-
| Green 10x FastDigest buffer || 3.0 μL || || || ||
+
| Green 10x FastDigest buffer || 3.0 μL  
|-
|-
-
| dH<sub>2</sub>O || 15 μL || || || ||
+
| dH<sub>2</sub>O || 15 μL  
|-
|-
-
| &nbsp; || 30 μL --> 37°C/ ~10 min. || || || ||
+
| &nbsp; || 30 μL --> 37°C/ ~10 min.  
|}
|}

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September 17, 2013

  • Restriction Digest of CMV promoter
    • for PEG DNA precipitation
Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.


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