Haynes Lab:Notebook/Engineering PC-TFs/2013/09/17

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(September 17, 2013)
(September 17, 2013)
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{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
{| {{table}} cellspacing="3" <!-- Digest rxn. table -->
|- valign="top"
|- valign="top"
 +
| bgcolor=#cfcfcf | '''Reagent'''
 +
| bgcolor=#cfcfcf | '''Volume'''
 +
| bgcolor=#cfcfcf | '''Reagent'''
 +
| bgcolor=#cfcfcf | '''Volume'''
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Reagent'''
| bgcolor=#cfcfcf | '''Volume'''
| bgcolor=#cfcfcf | '''Volume'''

Revision as of 00:01, 22 September 2013



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September 17, 2013

  • Restriction Digest of CMV promoter
    • for PEG DNA precipitation
Reagent Volume Reagent Volume Reagent Volume
DNA (plasmid) 10 μL
XbaI 1.0 μL
PstI 1.0 μL
Green 10x FastDigest buffer 3.0 μL
dH2O 15 μL
  30 μL --> 37°C/ ~10 min.


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