Haynes Lab:Notebook/Engineering PC-TFs/2013/09/12: Difference between revisions
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|style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | |style="background-color: #FFCC00"|<br>[[Image:Hayneslab3.gif|200px|center]][[Image:Asu logo 3.gif|200px|center]]<span style="font-size:22px;"><br></span> | ||
|style="background-color: #800000" align="center"| | |style="background-color: #800000" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==September 12, 2013== | ||
* Follow steps for gel electrophoresis. | * Follow steps for gel electrophoresis. | ||
* Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | * Orient top notch of gel electrophoresis apparatus. Place gel tray into larger tray. | ||
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| bgcolor=#cfcfcf | '''Reagent''' | | bgcolor=#cfcfcf | '''Reagent''' | ||
| bgcolor=#cfcfcf | '''Volume''' | | bgcolor=#cfcfcf | '''Volume''' | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:cmvgel2.jpg|250px| CMV and MV9 Stock digest]] [[Image:cmvgel1_cutout.jpg|250px|CMV and MV9 Stock Digest 3/18/2013]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| DNA (plasmid) || 20.0 μL | | DNA (plasmid) || 20.0 μL | ||
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| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. | ||
|} | |} | ||
''Zymoclean<sup>TM</sup> Gel DNA Recovery Kit'' | |||
*Add 3 volumes of ADB Buffer to each volume of gel (600 μL). | |||
*Incubate at 55°C for 10 minutes. | |||
*Centrifuge at ≥ 10,000 x g for 30 seconds. Discard flow-through. | |||
*Add 200 μL of DNA Wash Buffer to the column and centrifuge for 30 seconds. Repeat wash step. | |||
*Place Zymoclean Column into a new 1.5 mL tube. Add 20μL of dH<sub>2</sub>O to elute DNA | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
|- | |- | ||
! scope="col" style="background:#efefef;" | # | ! scope="col" style="background:#efefef;" | # | ||
! scope="col" style="background:#efefef;" | | ! scope="col" style="background:#efefef;" | Sequence | ||
! scope="col" style="background:#efefef;" | ng/µL | ! scope="col" style="background:#efefef;" | ng/µL | ||
|- | |- | ||
|1 | |1 | ||
| | |CMV | ||
| | | -1.17 | ||
|- | |- | ||
|2 | |2 | ||
| | |CMV | ||
| | |2.767 | ||
|- | |- | ||
|3 | |3 | ||
| | |CMV | ||
| | | -2.116 | ||
|- | |- | ||
| | |5 | ||
| | |MV9 | ||
| | |20.838 | ||
|- | |- | ||
|} | |} |
Latest revision as of 23:19, 26 September 2017
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September 12, 2013
ZymocleanTM Gel DNA Recovery Kit
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