Haynes Lab:Notebook/Engineering PC-TFs/2013/09/12: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(One intermediate revision by the same user not shown) | |||
Line 38: | Line 38: | ||
| || 30 μL --> 37°C/ ~10 min. | | || 30 μL --> 37°C/ ~10 min. | ||
|} | |} | ||
''Zymoclean<sup>TM</sup> Gel DNA Recovery Kit'' | |||
*Add 3 volumes of ADB Buffer to each volume of gel (600 μL). | |||
*Incubate at 55°C for 10 minutes. | |||
*Centrifuge at ≥ 10,000 x g for 30 seconds. Discard flow-through. | |||
*Add 200 μL of DNA Wash Buffer to the column and centrifuge for 30 seconds. Repeat wash step. | |||
*Place Zymoclean Column into a new 1.5 mL tube. Add 20μL of dH<sub>2</sub>O to elute DNA | |||
{|border="1" cellpadding="5" cellspacing="0" align="left" | {|border="1" cellpadding="5" cellspacing="0" align="left" | ||
Line 47: | Line 55: | ||
|1 | |1 | ||
|CMV | |CMV | ||
|-1.17 | | -1.17 | ||
|- | |- | ||
|2 | |2 | ||
Line 55: | Line 63: | ||
|3 | |3 | ||
|CMV | |CMV | ||
|-2.116 | | -2.116 | ||
|- | |- | ||
|5 | |5 |
Revision as of 21:19, 11 October 2013
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||
September 12, 2013
ZymocleanTM Gel DNA Recovery Kit
|