Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Summary)
(3/27/2013)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==3/27/2013==
==3/27/2013==
 +
 +
 +
*1. Kozak,V0120 + BL01
 +
*2. Kozak,V0120 + BL05
 +
*3. Kozak,V0120 + BL09
 +
*4. Kozak,V0120 + BL12
{| {{table}}
{| {{table}}

Revision as of 12:06, 2 April 2013

Engineering PC-TFs Main project page
Previous entry      Next entry

3/27/2013

  • 1. Kozak,V0120 + BL01
  • 2. Kozak,V0120 + BL05
  • 3. Kozak,V0120 + BL09
  • 4. Kozak,V0120 + BL12
1 2 3 4 5
DNA Insert1.41.82.02.5--
DNA Vector (Kozak)0.60.60.60.60.6
T4 Ligase11111
Lign Buffer (2x)55555
dH2O2.01.61.4.93.7
10 µL10 µL10 µL10 µL10 µL
  • In a .5 mL tube, pipette the ligation buffer first.
  • Then water, DNA insert, vector, and T4 ligase.
  • Once completed, allow them to incubate for 10 minutes.
  • Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes.
  • Then follow transformation process:



Personal tools