Haynes Lab:Notebook/Engineering PC-TFs/2013/03/27

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(Autocreate 2013/03/27 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
(Summary)
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==Summary==
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==3/27/2013==
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*  
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{| {{table}}
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| align="center" style="background:#0095B6;"|
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| align="center" style="background:#0095B6;"|'''1'''
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| align="center" style="background:#0095B6;"|'''2'''
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| align="center" style="background:#0095B6;"|'''3'''
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| align="center" style="background:#0095B6;"|'''4'''
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| align="center" style="background:#0095B6;"|'''5'''
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|-
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| DNA Insert||1.4||1.8||2.0||2.5||--
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|-
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| DNA Vector (Kozak)||0.6||0.6||0.6||0.6||0.6
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|-
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| T4 Ligase||1||1||1||1||1
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|-
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| Lign Buffer (2x)||5||5||5||5||5
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|-
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| dH2O||2.0||1.6||1.4||.9||3.7
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|-
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| ||10 µL||10 µL||10 µL||10 µL||10 µL
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|-
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|
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|}
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* In a .5 mL tube, pipette the ligation buffer first.
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* Then water, DNA insert, vector, and T4 ligase.
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* Once completed, allow them to incubate for 10 minutes.
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* Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes.
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* Then follow transformation process:

Revision as of 11:05, 2 April 2013

Engineering PC-TFs Main project page
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3/27/2013

1 2 3 4 5
DNA Insert1.41.82.02.5--
DNA Vector (Kozak)0.60.60.60.60.6
T4 Ligase11111
Lign Buffer (2x)55555
dH2O2.01.61.4.93.7
10 µL10 µL10 µL10 µL10 µL
  • In a .5 mL tube, pipette the ligation buffer first.
  • Then water, DNA insert, vector, and T4 ligase.
  • Once completed, allow them to incubate for 10 minutes.
  • Meanwhile, get ice bucket and allow competent E. coli cells to thaw for five minutes.
  • Then follow transformation process:



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