Haynes Lab:Notebook/Engineering PC-TFs/2013/03/25: Difference between revisions
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==Summary== | ==Summary== | ||
* Cutting inserts to be placed in mammalian vector | * Cutting inserts to be placed in mammalian vector | ||
A) Get biobricks and enzymes from freezer. | |||
PstI, SpeI | PstI, SpeI | ||
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{| {{table}} cellspacing="3" <!-- Digest rxn. table --> | {| {{table}} cellspacing="3" <!-- Digest rxn. table --> | ||
|- valign="top" | |- valign="top" | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | '''Reagent''' | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | '''Volume''' | ||
| rowspan="7" | [[Image:gel3_25_2013.jpg|200px|Stock digest 3/25/2013]] [[Image:gel3_25_2013_cutout.jpg|200px|Stock digest 3/18/2013]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:gel3_25_2013.jpg|200px|Stock digest 3/25/2013]] [[Image:gel3_25_2013_cutout.jpg|200px|Stock digest 3/18/2013]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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Revision as of 13:48, 26 March 2013
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Summary
A) Get biobricks and enzymes from freezer. PstI, SpeI The following list of parts are:
Follow steps for gel electrophoresis.
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