Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18: Difference between revisions
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<font size=3>''B)Follow digest procedure:'' | <font size=3>''B)Follow digest procedure:'' | ||
*DNA 20.0 µL | |||
*Enzyme 1 1.0 µL | |||
*Enzyme 2 1.0 µL | |||
*10x buffer 3.0 µL | |||
*dH2O 5.0 uL | |||
* = 30.0 uL </font> | |||
|} | |} | ||
Revision as of 20:33, 22 March 2013
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3/18/22Cutting inserts to be placed in mammalian vector
A) Get biobricks and enzymes from freezer. SpeI, PstI, XbaI The following list of parts are:
Digest Order: Biobrick Restriction Enzymes
Follow steps for gel electrophoresis.
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