Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18: Difference between revisions
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The following list of parts are: | The following list of parts are: | ||
* BL01 | * BL01 | ||
* BL05 | * BL05 | ||
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| rowspan="7" | [[Image:gel3_22_2013.jpg|200px|Stock digest 3/18/2013]] [[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/18/2013]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:gel3_22_2013.jpg|200px|Stock digest 3/18/2013]] [[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/18/2013]] <br>30 μL/lane, 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA (plasmid) || | | DNA (plasmid) || 15.0 μL | ||
|- | |||
| XbaI|| 1.0 μL | |||
|- | |- | ||
| | | PstI|| 1.0 μL | ||
|- | |- | ||
| | |10x FastDigest buffer + green loading dye || 1.0 μL | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 10 μL | ||
|- | |- | ||
| || 30 μL --> 37°C/ ~ | | || 30 μL --> 37°C/ ~10 min. | ||
|} | |} | ||
Revision as of 20:28, 22 March 2013
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SummaryCutting inserts to be placed in mammalian vector
A) Get biobricks and enzymes from freezer. SpeI, PstI, XbaI The following list of parts are:
Biobrick Restriction Enzymes
|