Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18: Difference between revisions
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== | ==3/18/22== | ||
Cutting inserts to be placed in mammalian vector | Cutting inserts to be placed in mammalian vector | ||
''' A) Get biobricks and enzymes from freezer.''' | ''' A) Get biobricks and enzymes from freezer.''' | ||
PstI, XbaI | |||
The following list of parts are: | The following list of parts are: | ||
* BL01 | * 1. BL01 | ||
* BL05 | * 2. BL05 | ||
* BL09 | * 3. BL09 | ||
* BL12 | * 4. BL12 | ||
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<font size=3>''B)Follow digest procedure:'' | <font size=3>''B)Follow digest procedure:'' | ||
*DNA 20.0 µL | |||
*Enzyme 1 1.0 µL | |||
*Enzyme 2 1.0 µL | |||
*10x buffer 3.0 µL | |||
*dH2O 5.0 uL | |||
* = 30.0 uL </font> | |||
|} | |} | ||
Revision as of 18:22, 6 September 2013
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3/18/22Cutting inserts to be placed in mammalian vector A) Get biobricks and enzymes from freezer. PstI, XbaI The following list of parts are:
Digest Order: Biobrick Restriction Enzymes
Follow steps for gel electrophoresis.
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