Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18

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[[Image:gel3_22_2013.jpg|200px|Stock digest 3/22/2013]]
[[Image:gel3_22_2013.jpg|200px|Stock digest 3/22/2013]]
[[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/22/2013]]
[[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/22/2013]]
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{|border="1" cellpadding="5" cellspacing="0" align="center"
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|-
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! scope="col" style="background:#efefef;" | #
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! scope="col" style="background:#efefef;" | Biobrick
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! scope="col" style="background:#efefef;" | ng/µL
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|-
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|1
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|hPCD : mCh : SP1AB (BL01)
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|18.834
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|-
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|2
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|fshPCD : mCh : SP1AB (BL05)
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|14.471
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|-
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|3
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|flyPCD : mCh : SP1A (BL09)
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|7.302
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|-
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|4
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|hPCD : BL01 (BL12)
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|10.84
 +
|-
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|}

Revision as of 23:09, 22 March 2013

Engineering PC-TFs Main project page
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Summary

Cutting inserts to be placed in mammalian vector

  • Procedure

A) Get biobricks and enzymes from freezer.

SpeI, PstI, XbaI

The following list of parts are:


  • BL01
  • BL05
  • BL09
  • BL12
Stock Digest

B)Follow digest procedure: 

  DNA          20.0 µL
  Enzyme 1     1.0  µL
  Enzyme 2     1.0  µL
  10x buffer   3.0  µL
  dH2O         5.0  uL
            
             = 30.0 uL


Digest Order:

Biobrick Restriction Enzymes

  • 1. BL01 XbaI/PstI
  • 2. BL05 XbaI/PstI
  • 3. BL09 XbaI/PstI
  • 4 BL12 XbaI/PstI


Stock digest 3/22/2013 Stock digest 3/22/2013


# Biobrick ng/µL
1 hPCD : mCh : SP1AB (BL01) 18.834
2 fshPCD : mCh : SP1AB (BL05) 14.471
3 flyPCD : mCh : SP1A (BL09) 7.302
4 hPCD : BL01 (BL12) 10.84



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