Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18

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(Summary)
(Summary)
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* BL12
* BL12
 +
{| {{table}}
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|align="center" style="background:#f0f0f1;"|'''Stock Digest'''
<font size=3>''B)Follow digest procedure:''
<font size=3>''B)Follow digest procedure:''
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   dH2O        5.0  uL
   dH2O        5.0  uL
              
              
-
               = 30.0 uL </font>
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               = 30.0 uL </font>  
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|}

Revision as of 21:59, 22 March 2013

Engineering PC-TFs Main project page
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Summary

Cutting inserts to be placed in mammalian vector

  • Procedure

A) Get biobricks and enzymes from freezer.

SpeI, PstI, XbaI

The following list of parts are:


  • BL01
  • BL05
  • BL09
  • BL12
Stock Digest

B)Follow digest procedure:

  DNA          20.0 µL
  Enzyme 1     1.0  µL
  Enzyme 2     1.0  µL
  10x buffer   3.0  µL
  dH2O         5.0  uL
            
             = 30.0 uL  


Digest Order:

Biobrick Restriction Enzymes

  • 1. BL01 XbaI/PstI
  • 2. BL05 XbaI/PstI
  • 3. BL09 XbaI/PstI
  • 4 BL12 XbaI/PstI


Stock digest 3/22/2013 Stock digest 3/22/2013



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