Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18: Difference between revisions
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==Summary== | ==Summary== | ||
* | Cutting inserts to be placed in mammalian vector | ||
* '''Procedure''' | |||
''' A) Get biobricks and enzymes from freezer.''' | |||
SpeI, PstI, XbaI | |||
The following list of parts are: | |||
* BL01 | |||
* BL05 | |||
* BL09 | |||
* BL12 | |||
<font size=3>''B)Follow digest procedure:'' | |||
DNA 20.0 µL | |||
Enzyme 1 1.0 µL | |||
Enzyme 2 1.0 µL | |||
10x buffer 3.0 µL | |||
dH2O 5.0 uL | |||
= 30.0 uL </font> | |||
''Digest Order:'' | |||
Biobrick Restriction Enzymes | |||
*1. BL01 XbaI/PstI | |||
*2. BL05 XbaI/PstI | |||
*3. BL09 XbaI/PstI | |||
*4 BL12 XbaI/PstI | |||
[[Image:gel3_22_2013.jpg|200px|Stock digest 3/22/2013]] | |||
[[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/22/2013]] | |||
Revision as of 19:54, 22 March 2013
 Engineering PC-TFs
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SummaryCutting inserts to be placed in mammalian vector
A) Get biobricks and enzymes from freezer. SpeI, PstI, XbaI The following list of parts are:
B)Follow digest procedure: DNA 20.0 µL
Enzyme 1 1.0 µL
Enzyme 2 1.0 µL
10x buffer 3.0 µL
dH2O 5.0 uL
= 30.0 uL
Biobrick Restriction Enzymes
| |
