Haynes Lab:Notebook/Engineering PC-TFs/2013/03/18

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/03/18 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
(Summary)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Summary==
==Summary==
-
*  
+
Cutting inserts to be placed in mammalian vector
 +
* '''Procedure'''
 +
 
 +
''' A) Get biobricks and enzymes from freezer.'''
 +
 
 +
SpeI, PstI, XbaI
 +
 
 +
The following list of parts are:
 +
 
 +
 
 +
* BL01
 +
* BL05
 +
* BL09
 +
* BL12
 +
 
 +
<font size=3>''B)Follow digest procedure:''
 +
 
 +
  DNA          20.0 µL
 +
  Enzyme 1    1.0  µL
 +
  Enzyme 2    1.0  µL
 +
  10x buffer  3.0  µL
 +
  dH2O        5.0  uL
 +
           
 +
              = 30.0 uL </font>
 +
 
 +
 
 +
''Digest Order:''
 +
 
 +
Biobrick    Restriction Enzymes
 +
*1. BL01      XbaI/PstI
 +
*2. BL05      XbaI/PstI
 +
*3. BL09      XbaI/PstI
 +
*4  BL12      XbaI/PstI
 +
 
 +
 
 +
[[Image:gel3_22_2013.jpg|200px|Stock digest 3/22/2013]]
 +
[[Image:gel3_22_2013_cutout.jpg|200px|Stock digest 3/22/2013]]

Revision as of 21:54, 22 March 2013

Engineering PC-TFs Main project page
Previous entry      Next entry

Summary

Cutting inserts to be placed in mammalian vector

  • Procedure

A) Get biobricks and enzymes from freezer.

SpeI, PstI, XbaI

The following list of parts are:


  • BL01
  • BL05
  • BL09
  • BL12

B)Follow digest procedure:

  DNA          20.0 µL
  Enzyme 1     1.0  µL
  Enzyme 2     1.0  µL
  10x buffer   3.0  µL
  dH2O         5.0  uL
            
             = 30.0 uL 


Digest Order:

Biobrick Restriction Enzymes

  • 1. BL01 XbaI/PstI
  • 2. BL05 XbaI/PstI
  • 3. BL09 XbaI/PstI
  • 4 BL12 XbaI/PstI


Stock digest 3/22/2013 Stock digest 3/22/2013



Personal tools