Haynes Lab:Notebook/Engineering PC-TFs/2013/03/05

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(→Summary)
(→Summary)
Line 41: Line 41:
[[Image:gel3_5_2013.jpg|500px|thumb|Stock digest 3/5/2013]]
[[Image:gel3_5_2013.jpg|500px|thumb|Stock digest 3/5/2013]]
[[Image:gel3_5_2013_cutout.jpg|500px|thumb|left|Stock digest 3/5/2013]]
[[Image:gel3_5_2013_cutout.jpg|500px|thumb|left|Stock digest 3/5/2013]]
-
 
+
<br>
-
<fontsize=4>''Transformation of hPCD, fshPCD, flyPCD''
+
*<font size=4>''Transformation of hPCD, fshPCD, flyPCD''

Revision as of 23:11, 5 March 2013

Engineering PC-TFs Main project page
Previous entry      Next entry

Summary

  • Procedure

A) Get biobricks and enzymes from freezer.

SpeI, PstI, XbaI

The following list of parts are:


  • BL01
  • BL05
  • BL09

B)Follow digest procedure: 

  DNA          20.0 µL
  Enzyme 1     1.0  µL
  Enzyme 2     1.0  µL
  10x buffer   3.0  µL
  dH2O         5.0  uL
            
             = 30.0 uL


Digest Order:

Biobrick Restriction Enzymes

  • 1. BL01 XbaI/PstI
  • 2. BL05 XbaI/PstI
  • 3. BL09 XbaI/PstI


Stock digest 3/5/2013
Stock digest 3/5/2013
Stock digest 3/5/2013
Stock digest 3/5/2013


  • Transformation of hPCD, fshPCD, flyPCD


Retrieved from "http://openwetware.org/wiki/Haynes_Lab:Notebook/Engineering_PC-TFs/2013/03/05"
Personal tools