Haynes Lab:Notebook/Engineering PC-TFs/2013/03/05: Difference between revisions

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SpeI, PstI, XbaI
SpeI, PstI, XbaI


The following list of biobricks are:
The following list of parts are:




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               = 30.0 uL
               = 30.0 uL


[[Image:back_insert.jpg|500px|thumb|right|Prep for Assembly]]
 
''Digest Order:''
''Digest Order:''


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[[Image:gel3_5_2013.jpg|500px|thumb|Stock digest 3/5/2013]]


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Revision as of 19:55, 5 March 2013

Engineering PC-TFs <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Summary

  • Procedure

A) Get biobricks and enzymes from freezer.

SpeI, PstI, XbaI

The following list of parts are:


  • BL01
  • BL05
  • BL09

B)Follow digest procedure: 

  DNA          20.0 µL
  Enzyme 1     1.0  µL
  Enzyme 2     1.0  µL
  10x buffer   3.0  µL
  dH2O         5.0  uL
            
             = 30.0 uL


Digest Order:

Biobrick Restriction Enzymes

  • 1. BL01 XbaI/PstI
  • 2. BL05 XbaI/PstI
  • 3. BL09 XbaI/PstI


Stock digest 3/5/2013


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