Haynes Lab:Notebook/Engineering PC-TFs/2013/03/05

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(Autocreate 2013/03/05 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs)
(Summary)
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==Summary==
==Summary==
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*  
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* '''Procedure'''
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''' A) Get biobricks and enzymes from freezer.'''
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SpeI, PstI, XbaI
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The following list of biobricks are:
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 +
 
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* BL01
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* BL05
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* BL09
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''B)Follow digest procedure:''
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  DNA          20.0 µL
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  Enzyme 1    1.0  µL
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  Enzyme 2    1.0  µL
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  10x buffer  3.0  µL
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  dH2O        5.0  uL
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              = 30.0 uL
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[[Image:back_insert.jpg|500px|thumb|right|Prep for Assembly]]
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''Digest Order:''
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Biobrick    Restriction Enzymes
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*1. BL01      XbaI/PstI
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*2. BL05      XbaI/PstI
 +
*3. BL09      XbaI/PstI
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Revision as of 21:54, 5 March 2013

Engineering PC-TFs Main project page
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Summary

  • Procedure

A) Get biobricks and enzymes from freezer.

SpeI, PstI, XbaI

The following list of biobricks are:


  • BL01
  • BL05
  • BL09

B)Follow digest procedure:

  DNA          20.0 µL
  Enzyme 1     1.0  µL
  Enzyme 2     1.0  µL
  10x buffer   3.0  µL
  dH2O         5.0  uL
            
             = 30.0 uL
Prep for Assembly
Prep for Assembly

Digest Order:

Biobrick Restriction Enzymes

  • 1. BL01 XbaI/PstI
  • 2. BL05 XbaI/PstI
  • 3. BL09 XbaI/PstI




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