Haynes Lab:Notebook/Engineering PC-TFs/2013/03/05
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==Summary== | ==Summary== | ||
| - | * | + | * '''Procedure''' |
| + | |||
| + | ''' A) Get biobricks and enzymes from freezer.''' | ||
| + | |||
| + | SpeI, PstI, XbaI | ||
| + | |||
| + | The following list of biobricks are: | ||
| + | |||
| + | |||
| + | * BL01 | ||
| + | * BL05 | ||
| + | * BL09 | ||
| + | |||
| + | ''B)Follow digest procedure:'' | ||
| + | |||
| + | DNA 20.0 µL | ||
| + | Enzyme 1 1.0 µL | ||
| + | Enzyme 2 1.0 µL | ||
| + | 10x buffer 3.0 µL | ||
| + | dH2O 5.0 uL | ||
| + | |||
| + | = 30.0 uL | ||
| + | |||
| + | [[Image:back_insert.jpg|500px|thumb|right|Prep for Assembly]] | ||
| + | ''Digest Order:'' | ||
| + | |||
| + | Biobrick Restriction Enzymes | ||
| + | *1. BL01 XbaI/PstI | ||
| + | *2. BL05 XbaI/PstI | ||
| + | *3. BL09 XbaI/PstI | ||
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Revision as of 22:54, 5 March 2013
 Engineering PC-TFs
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Summary
A) Get biobricks and enzymes from freezer. SpeI, PstI, XbaI The following list of biobricks are:
B)Follow digest procedure: DNA 20.0 µL
Enzyme 1 1.0 µL
Enzyme 2 1.0 µL
10x buffer 3.0 µL
dH2O 5.0 uL
= 30.0 uL
 Digest Order: Biobrick Restriction Enzymes
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