Haynes Lab:Notebook/Engineering PC-TFs/2013/03/05: Difference between revisions
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Line 20: | Line 20: | ||
* BL09 | * BL09 | ||
''B)Follow digest procedure:'' | <font size=3>''B)Follow digest procedure:'' | ||
DNA 20.0 µL | DNA 20.0 µL | ||
Line 28: | Line 28: | ||
dH2O 5.0 uL | dH2O 5.0 uL | ||
= 30.0 uL | = 30.0 uL </font> | ||
Line 42: | Line 42: | ||
[[Image:gel3_5_2013_cutout.jpg|200px|Stock digest 3/5/2013]] | [[Image:gel3_5_2013_cutout.jpg|200px|Stock digest 3/5/2013]] | ||
<br> | <br> | ||
<font size=4>''Transformation of hPCD, fshPCD, flyPCD'' <font | <font size=4>''Transformation of hPCD, fshPCD, flyPCD''</font> | ||
<br> | <br> | ||
*Warm 100 μg/mL Amp agar plates at 37 °C | *Warm 100 μg/mL Amp agar plates at 37 °C |
Revision as of 20:19, 5 March 2013
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Summary
A) Get biobricks and enzymes from freezer. SpeI, PstI, XbaI The following list of parts are:
B)Follow digest procedure: DNA 20.0 µL Enzyme 1 1.0 µL Enzyme 2 1.0 µL 10x buffer 3.0 µL dH2O 5.0 uL = 30.0 uL
Biobrick Restriction Enzymes
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