Haynes Lab:Notebook/Engineering PC-TFs/2013/03/02: Difference between revisions
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==Summary== | ==Summary== | ||
* Retrieve | |||
<font size=3>''E/P Digest for FURI 2013-2014 application''</font> | |||
* Retrieve BL01, BL05, BL09, BL011 assembly from freezer and thaw at room temperature. | |||
* Also let restriction enzymes thaw. | * Also let restriction enzymes thaw. | ||
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| align="center" style="background:#f0f0f0;"|'''Master Mix''' | | align="center" style="background:#f0f0f0;"|'''Master Mix''' | ||
|- | |- | ||
| ||||1 rxn||x | | ||||1 rxn||x 4 | ||
|- | |- | ||
| DNA plasmid||||3||-- | | DNA plasmid||||3||-- | ||
|- | |- | ||
| enzyme 1||||1|| | | enzyme 1||||1||4 | ||
|- | |- | ||
| enzyme 2||||1|| | | enzyme 2||||1||4 | ||
|- | |- | ||
| 10x buffer||||1.5|| | | 10x buffer||||1.5||6 | ||
|- | |- | ||
| dH2O||||8.5|| | | dH2O||||8.5||34 | ||
|- | |- | ||
| | | | ||
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* Pour gel into tray. | * Pour gel into tray. | ||
* Wash the agarose gel flask. | * Wash the agarose gel flask. | ||
* Run gel for 30 min at 100 V. Check gel under UV light. | |||
Revision as of 19:01, 2 March 2013
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SummaryE/P Digest for FURI 2013-2014 application
Follow steps for gel electrophoresis.
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