Haynes Lab:Notebook/Engineering PC-TFs/2013/02/09
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< Haynes Lab:Notebook | Engineering PC-TFs | 2013 | 02(Difference between revisions)
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| - | == | + | ==2/9/2013== |
| - | * Type IIS assembly - | + | * Type IIS assembly - PCR products |
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| - | | rowspan="7" | <u>Expected:</u><br>1. pSB1A3 = ~2000<br>2. hPCD = 186<br>3. BL02 = 1431<br> 4. BL03 = 1731<br> 5. BL04 = 1686 | + | | rowspan="7" | <u>Expected:</u><br>1. pSB1A3 = ~2000<br>2. hPCD = 186<br>3. BL02 = 1431<br> 4. BL03 = 1731<br> 5. BL04 = 1686 |
| rowspan="7" | [[Image:gg_gel2-9-2013.jpg|200px|PCR results]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:gg_gel2-9-2013.jpg|200px|PCR results]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
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| - | Purification (Completed on | + | Purification (Completed on 2-09-2013) |
| - | * Clean with Zymo DNA | + | * Clean with Zymo DNA clean &concentrator kit; eluted w/ 20 μL dH<sub>2</sub>O |
* Measure [DNA] (1-29-2013) | * Measure [DNA] (1-29-2013) | ||
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2/9/2013
PCR
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