Haynes Lab:Notebook/Engineering PC-TFs/2013/02/05 - Revision history

Brady S. Laughlin: /* 2/5/13 */

Brady S. Laughlin — Sat, 09 Feb 2013 20:05:19 GMT

2/5/13

←Older revision		Revision as of 20:05, 9 February 2013
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	* Check results tomorrow.		* Check results tomorrow.

-	[[plates_1_5_2013.jpg\|400px\|\|left\|]]	+	[[Image:plates_1_5_2013.jpg\|400px\|\|left\|]]

Brady S. Laughlin: /* 2/5/13 */

Brady S. Laughlin — Sat, 09 Feb 2013 20:04:43 GMT

2/5/13

←Older revision		Revision as of 20:04, 9 February 2013
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	* Plate cells on pre-warmed LB agar + antibiotic. (''I plated 100 μL of the cells. Then put beads on plates and shook.) Protocol didn't specify.'') Grow overnight at 37°C.		* Plate cells on pre-warmed LB agar + antibiotic. (''I plated 100 μL of the cells. Then put beads on plates and shook.) Protocol didn't specify.'') Grow overnight at 37°C.
	* Check results tomorrow.		* Check results tomorrow.
		+
		+	[[plates_1_5_2013.jpg\|400px\|\|left\|]]

Brady S. Laughlin: /* Summary */

Brady S. Laughlin — Sat, 09 Feb 2013 20:01:34 GMT

Summary

←Older revision		Revision as of 20:01, 9 February 2013
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Summary~~==	+	==2/5/13==
-	*	+	<font size=3>'''Bacterial transformation'''</font>
		+	* Added total volume (10.0 μL) to 50 μL chemically competent cells (e.g., BL21) in a 2.0 mL tube.
		+	* Incubated on ice for 2 min., heat shock at 42°C for exactly 45 sec., and placed on ice.
		+	* Added 800 μL sterile SOC medium.
		+	* Grow with shaking at 37°C for 30 min.(''Taped tubes to the shaker rack.'')
		+	* Pelleted the cells at top speed in a microcentrifuge for 3 min. at room temperature.
		+	* Discarded the supernatant. Resuspended the cells in 100 μL LB + antibiotic.
		+	* Plate cells on pre-warmed LB agar + antibiotic. (''I plated 100 μL of the cells. Then put beads on plates and shook.) Protocol didn't specify.'') Grow overnight at 37°C.
		+	* Check results tomorrow.
		+
		+
		+

Brady S. Laughlin: Autocreate 2013/02/05 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs

Brady S. Laughlin — Sat, 09 Feb 2013 19:58:31 GMT

Autocreate 2013/02/05 Entry for Haynes_Lab:Notebook/Engineering_PC-TFs

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Engineering PC-TFs</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Summary==
*


|}

__NOTOC__