Haynes Lab:Notebook/Engineering PC-TFs/2013/01/29: Difference between revisions
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* The volume of purified DNA (x) you will need to dilute in a final volume of 20 μL = 20 μL final volume * 20 fmols/μL * '''length in bp''' * 650 fg/fmol ÷ 1,000,000 fg/ng ÷ '''measured ng/μL''' | * The volume of purified DNA (x) you will need to dilute in a final volume of 20 μL = 20 μL final volume * 20 fmols/μL * '''length in bp''' * 650 fg/fmol ÷ 1,000,000 fg/ng ÷ '''measured ng/μL''' | ||
** Formula: x = '''length in bp''' ÷ '''measured ng/μL''' * 0.26 | ** Formula: x = '''length in bp''' ÷ '''measured ng/μL''' * 0.26 | ||
* <font size=3>Purified PCR Product to 20fmol/μL | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Sample | |||
| bgcolor=#cfcfcf | μL of Sample | |||
| bgcolor=#cfcfcf | μL of dH2O | |||
|- | |||
| 1. pSB1A3 || 7.8|| 12.2 | |||
|- | |||
| 2. hPCD || 2.8 || 17.2 | |||
|- | |||
| 3. BL02 || 6.4|| 13.6 | |||
|- | |||
| 4. BL03 || 5.5 || 14.5 | |||
|- | |||
| 5. BL04 || 8.8|| 11.2 | |||
|- | |||
| 6. fshPCD || 2.3|| 7.7 | |||
|} | |||
* '''Perform BsmBI/ T4 ligase mediated assembly''' | * '''Perform BsmBI/ T4 ligase mediated assembly''' |
Revision as of 00:51, 1 February 2013
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Summary
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