Haynes Lab:Notebook/Engineering PC-TFs/2013/01/25: Difference between revisions
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* All of the reactions are as follows: | * All of the reactions are as follows: | ||
# | # pSB1A3, gg0001, gg0002 | ||
# | # hPCD, gg0003, gg0004 | ||
# | # BL02, gg0005, gg0007 | ||
# | # BL03, gg0005, gg0008 | ||
# BL04, gg0005, gg0009 | |||
# fshPCD, gg0010, gg0012 | |||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. pSB1A3 = ~2000<br>2. hPCD = 186<br>3. | | rowspan="7" | <u>Expected:</u><br>1. pSB1A3 = ~2000<br>2. hPCD = 186<br>3. BL02 = 1431<br> 4. BL03 = 1731<br> 5. BL04 = 1686<br> 6.fshPCD = 186<br> | ||
| rowspan="7" | [[Image: | | rowspan="7" | [[Image:gg_gel1-25-2013.jpg|200px|PCR results]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
| DNA(plasmid) || 0.2 μL | | DNA(plasmid) || 0.2 μL | ||
Line 43: | Line 45: | ||
|} | |} | ||
* 95°C, 3 min. | * 95°C, 3 min. | ||
* [95°C, 30 sec.; 57°C, 30 sec.; 72°C, 1 min.] x35 | * [95°C, 30 sec.; 57°C, 30 sec.; 72°C, 1 min.] x35 | ||
* 72°C, 3 min. | * 72°C, 3 min. | ||
* 4°C, ∞ | * 4°C, ∞ | ||
Purification (Completed on 1-25-2013) | |||
* Clean with Zymo DNA c&c; elute w/ 20 μL dH<sub>2</sub>O | |||
* Measure [DNA] (1-29-2013) | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | |||
|- valign="top" | |||
| bgcolor=#cfcfcf | Sample | |||
| bgcolor=#cfcfcf | OD 260 | |||
| bgcolor=#cfcfcf | 260/280 | |||
| bgcolor=#cfcfcf | ng/μL | |||
|- | |||
| 1. pSB1A3 || .084|| 1.801 || 83.954 | |||
|- | |||
| 2. hPCD || .017 || 1.734|| 17.344 | |||
|- | |||
| 3. BL02 || .058 || 1.764|| 58.413 | |||
|- | |||
| 4. BL03 || .082 || 1.831|| 81.877 | |||
|- | |||
| 5. BL04 || .05 || 1.888 || 49.561 | |||
|- | |||
| 6. fshPCD || .021 || 1.622 || 21.378 | |||
|} | |||
Revision as of 23:52, 31 January 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||
01/25/13
PCR
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