Haynes Lab:Notebook/Engineering PC-TFs/2013/01/25: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(2 intermediate revisions by the same user not shown) | |||
Line 18: | Line 18: | ||
# pSB1A3, gg0001, gg0002 | # pSB1A3, gg0001, gg0002 | ||
# | # hPCD, gg0003, gg0004 | ||
# | # BL02, gg0005, gg0007 | ||
# | # BL03, gg0005, gg0008 | ||
# BL04, gg0005, gg0009 | |||
# fshPCD, gg0010, gg0012 | |||
Line 27: | Line 29: | ||
| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. pSB1A3 = ~2000<br>2. hPCD = 186<br>3. | | rowspan="7" | <u>Expected:</u><br>1. pSB1A3 = ~2000<br>2. hPCD = 186<br>3. BL02 = 1431<br> 4. BL03 = 1731<br> 5. BL04 = 1686<br> 6.fshPCD = 186<br> | ||
| rowspan="7" | [[Image:gg_gel1-25-2013.jpg|200px|PCR results]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:gg_gel1-25-2013.jpg|200px|PCR results]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- | ||
Line 49: | Line 51: | ||
Purification | Purification (Completed on 1-25-2013) | ||
* Clean with Zymo DNA c&c; elute w/ 20 μL dH<sub>2</sub>O | * Clean with Zymo DNA c&c; elute w/ 20 μL dH<sub>2</sub>O | ||
* Measure [DNA] | |||
* Measure [DNA] (1-29-2013) | |||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
|- valign="top" | |- valign="top" |
Revision as of 22:35, 27 January 2013
Engineering PC-TFs | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||
01/25/13
PCR
|