Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10

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Summary

  • Gibson PCR



PCR of Gibson BioBricks

  • ---Karmella 13:55, 10 December 2012 (EST): Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions. Elute with 30 μL H2O. Measure concentration on the plate reader.
  • ---Karmella 13:55, 10 December 2012 (EST): Give me your pSB1A3 so that I can do a retransformation



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