Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10

(Difference between revisions)

Revision as of 16:15, 10 December 2012

Engineering PC-TFs

PCR of Gibson BioBricks

---Karmella 13:55, 10 December 2012 (EST): Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions. Elute with 30 μL H₂O. Measure concentration on the plate reader.
---Karmella 13:55, 10 December 2012 (EST): Give me your pSB1A3 so that I can do a retransformation

@@ Line 13: / Line 13: @@
 '''PCR of Gibson BioBricks'''
-* *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions. Elute with 30 μL H<sub>2</sub>O. Measure concentration on the plate reader.
+*'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions. Elute with 30 μL H<sub>2</sub>O. Measure concentration on the plate reader.
-* *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Give me your pSB1A3 so that I can do a retransformation
+*'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Give me your pSB1A3 so that I can do a retransformation