Haynes Lab:Notebook/Engineering PC-TFs/2012/12/10
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'''PCR of Gibson BioBricks''' | '''PCR of Gibson BioBricks''' | ||
| - | * *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions. | + | * *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Do not gel purify PCR products. Instead, Zymo DNA Clean & Concentrator kit to clean up the PCR reactions. Elute with 30 μL H<sub>2</sub>O. Measure concentration on the plate reader. |
* *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Give me your pSB1A3 so that I can do a retransformation | * *'''[[User:Karmella Haynes|---Karmella]] 13:55, 10 December 2012 (EST)''': Give me your pSB1A3 so that I can do a retransformation | ||
Revision as of 14:58, 10 December 2012
 Engineering PC-TFs
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Summary
PCR of Gibson BioBricks
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